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Abstract
Next Generation Sequencing allows whole genome analysis of small RNAs at an unprecedented level. Current technologies allow for the generation of 200 million data points in a single instrument run. In addition to allowing for the complete characterization of all known small RNAs in a sample, these applications are also ideal for the identification of novel small RNAs. This presentation will provide an overview of micro RNA expression analysis from raw data to biological significance using examples from publicly available datasets and Geospiza’s GeneSifter software.
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