Monday, June 22, 2009

Sneak Peak: RNA-Seq - Global Profiling of Gene Activity and Alternative Splicing

Join us June 30 at 10:00 am PDT. Eric Olson, Geospiza's VP of Product Development will present an interesting webinar on using RNA-Seq to measure gene expression and discover alternatively spliced messages using GeneSifter Analysis Edition.


Next Generation Sequencing applications such as RNA-Seq, Tag Profiling and Small RNA Analysis allow whole genome analysis of coding as well as non-coding RNA at an unprecedented level. Current technologies allow for the generation of 200 million data points in a single instrument run. In addition to allowing for the complete characterization of all known RNAs in a sample, these applications are also ideal for the identification of novel RNAs and novel splicing events for known RNAs.

This presentation will provide an overview of the RNA-Seq data analysis process with emphasis on calculating gene and exon level expression values as well as identifying splice junctions from short read data. Comparisons of multiple groups to identify differential gene expression as well as differential splicing will also be discussed. Using data drawn from the GEO data repository and Short Read Archive (SRA), analysis examples will be presented for both Illumina’s GA and ABI’s SOLiD instruments.

To register visit the Geospiza webex event page.

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