RNA sequencing is one of the most popular Next Generation Sequencing (NGS) applications. Next Thursday, February 11 at 10:00 A.M. PDT (1:00 P.M. EDT), we kick off our 2010 webinar series with a presentation designed to help you understand whole transcriptome data analysis and what can be learned in these experiments. In addition, we will show off some of our latest tools and interfaces that can be used to discover new RNAs, new splice forms of transcripts, and alleles of expressed genes.
Summary
RNA sequencing applications such as Whole Transcriptome Analysis, Tag Profiling and Small RNA Analysis allow whole genome analysis of coding as well as non-coding RNA at an unprecedented level. Current technologies allow for the generation of 500 million data points in a single instrument run. In addition to allowing for the complete characterization of all known RNAs in a sample (gene level expression summaries, exon usage, splice junction, single nucleotide variants, insertions and deletions), these applications are also ideal for the identification of novel RNAs as well as novel splicing events.
This presentation will provide an overview of Whole Transcriptome data analysis workflows with emphasis on calculating gene and exon level expression values as well as identifying splice junctions and variants from short read data. Comparisons of multiple groups to identify differential gene expression as well as differential splicing will also be discussed. Using data drawn from the GEO data repository and Short Read Archive (SRA), analysis examples will be presented for both Illumina’s GA and Lifetech’s SOLiD instruments.
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